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‎ 10.29252/sjfst.2.1.1

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Rezaei E. Level of Expression of BDNF and TNF-Alpha in the Somatosensory Cortex and its Effect on Social Interaction in Autism Model Rats Induced by Valproic Acid. sjfst 2020; 2 (1) :1-13
URL: http://sjfst.srpub.org/article-6-46-fa.html
رضایی الهه. بررسی سطح بیان BDNF و TNF-Alpha در قشر حسی مغز در اثر القای اسید والپروئیک و تأثیر آن بر تعامل اجتماعی در موشهای صحرایی مدل اوتیسم. تحقیقات بنیادی علوم و تکنولوژی. 1398; 2 (1) :1-13

URL: http://sjfst.srpub.org/article-6-46-fa.html

بررسی سطح بیان BDNF و TNF-Alpha در قشر حسی مغز در اثر القای اسید والپروئیک و تأثیر آن بر تعامل اجتماعی در موشهای صحرایی مدل اوتیسم
الهه رضایی*
دکترای زیست شناسی جانوری
چکیده:   (8951 مشاهده)
مطالعات نشان داده است که قرار گرفتن در معرض اسید والپروئیک (VPA) در دوران بارداری ممکن است منجر به "سندرم والپروات جنین" شود که دارای خصوصیاتی شبیه به اوتیسم است. هدف از این مطالعه بررسی سطح بیان فاکتور نوروتروفیک مشتق شده از مغز، فاکتور نکروز تومور آلفا و تأثیر این عوامل بر قشر مغز در موشهای صحرایی مدل اوتیسم با ااستفاده از اثر القایی اسید والپروئیک در دوره قبل از تولد و همچنین عملکردهای اجتماعی و اختلالات رفتاری مرتبط با مدلهای حیوانی اوتیسم در موشها است. در اواسط روز دوازدهم بارداری (ED 12.5)  ، میران 500 میلی گرم اسید والپروئیک (VPA ، SIGMA) محلول در بافر فسفات (PBS) به صورت داخل صفاقی به موشهای ماده در گروه آزمایش تزریق شد (10 =n). 30 و 60 روز پس از تولد ، برای ارزیابی رفتار اجتماعی و  تعامل اجتماعی ، آزمون های میدان باز و آزمایش های Y-Maze انجام شد. سپس، سطح بیان BDNF و TNF-α قشر حسی مغز با روش ELISA  در سرم اندازه گیری شد. نتایج این آزمایشات نشان داد که  در موشهای صحرایی مدل اوتیسم در اثر القای اسید والپروئیک ، ارتباطات اجتماعی ضعیفی وجود دارد و به دنبال آن کاهش و افزایش سیتوکینهایی مانند BDNF و TNF-alpha در قشر حسی و سرم خون موشها مشاهده گردید. چنین تجزیه و تحلیل هایی حداقل در مدل های حیوانی راهی برای شناخت اختلال اجتماعی ناشی از اوتیسم است.
واژه‌های کلیدی: اوتیسم، قشر حسی مغز، فاکتور نوروتروفیک مشتق از مغز، فاکتور نکروز تومور آلفا، آزمون رفتار اجتماعی
متن کامل [PDF 748 kb]   (753 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: ژنتیک
دریافت: 1398/7/16 | ویرایش نهایی: 1398/9/22 | پذیرش: 1398/10/4 | انتشار: 1398/11/11
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